Potential of miR-25-3p in protection of chondrocytes: emphasis on osteoarthritis

Introduction. Osteoarthritis (OA) is the most prevailing musculoskeletal dysfunction triggered by lesions in synovial membranes and articular cartilage. MicroRNAs (miRNAs) have emerged as crucial regulators participated many biological processes, such osteoarthritis. This study was undertaken to address role of miR-25-3p apoptosis rat chondrocytes under an OA-like condition its underlying mechanism. Material methods. OA cellular model established TNF-a induction. Then, qRTPCR Western blotting were utilized for evaluation expressions insulin-like growth factor-binding protein 7 (IGFBP7), CCK-8 assay inspection chondrocyte viability, flow cytometry assessment cell rate, detection cleaved caspase-3 level dual-luciferase reporter gene verification targeting relationship between IGFBP7. Results. The expression decreased IGFBP7 elevated TNF-a-induced chondrocytes. inhibited promoted evidenced enhanced viability suppressed after overexpression or knockdown. facilitated repressed negatively regulating Conclusions. MiR-25-3p regulates promote proliferation restrain apoptosis. Our findings suggest that regulation may emerge a novel therapeutic regimen OA.